Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181151_wukp GTGCTTGCTGCCAGGTTTGACCACGTGAGTTG(+AGGACA)GATCCCTGGAACCCACATGGTGGAAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613181149_by7e CAATTCTCTCTTTCCACCATGTGGGTTCCAGG(+AGGACA)GATCCAACTCACGTGGTCAAACCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613181148_2tg8 GTGGTCATTGAGTGCCACCAGTACATCTGATG(+AGGACA)GATCCAGTTGGCACGTCATGACAGATTGACT NGG 10 8 17 8 24 0 100 5 10
    240613181147_22az ACACAAGTCAATCTGTCATGACGTGCCAACTG(+AGGACA)GATCCATCAGATGTACTGGTGGCACTCAATG NGG 10 8 17 8 24 0 100 5 10
    240613181145_ubzi AGGACGGAGCCTAGGACTCTGTACCTGCCAAG(+AGGACA)GATCCCAGAGCCCAAGTATGTGGGGCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613181143_rczm CCAAACCAAAGCCCCACATACTTGGGCTCTGG(+AGGACA)GATCCTTGGCAGGTACAGAGTCCTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613181140_3mp8 AAGAAAAGGAGCATGAAAAACCATATCCCAGG(+AGGACA)GTACCTTTTCATGAGTTGATGTCCCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613181139_0kj7 TATTGGTGGAAGGGACATCAACTCATGAAAAG(+AGGACA)GTACCCTGGGATATGGTTTTTCATGCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613181137_q7pe GGGAGGTCTGAAAGTAATTGCTACAAAAGTTG(+AGGACA)GTACCTAAAAATGCTGTGGTGCCCCACAGAA NGG 10 8 17 8 24 0 100 5 10
    240613181136_qjz1 CATATTTCTGTGGGGCACCACAGCATTTTTAG(+AGGACA)GTACCAACTTTTGTAGCAATTACTTTCAGAC NGG 10 8 17 8 24 0 100 5 10