Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181056_z5b3 ATTTGGATTTAAATGGATTTGAAAGTCTTTGG(+AGGACA)GATCCTTGAAGGATTTGTAATAATCACTTTC NGG 10 8 17 8 24 0 100 5 10
    240613181055_ipul TTAGAGAAAGTGATTATTACAAATCCTTCAAG(+AGGACA)GATCCCAAAGACTTTCAAATCCATTTAAATC NGG 10 8 17 8 24 0 100 5 10
    240613181052_57mm GGCCCCGGTAATTAAGAGGTGAAATTCGCCTG(+AGGACA)GTACCAAGTGGGGCTGTGAACTTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240613181051_my4c AATTATTTAATTTAAGTTCACAGCCCCACTTG(+AGGACA)GTACCAGGCGAATTTCACCTCTTAATTACCG NGG 10 8 17 8 24 0 100 5 10
    240613181049_92ld TTTCAAGTATCGTGATGGATGAAGATGGGGGG(+AGGACA)GATCCTTCGCTTACATCTCCACACTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613181044_kqir CTGAAAACCAAAGTGTGGAGATGTAAGCGAAG(+AGGACA)GATCCCCCCCATCTTCATCCATCACGATACT NGG 10 8 17 8 24 0 100 5 10
    240613181043_zo1y TTGAGCTCAAGTGAATTGACTTTTTTTTGACG(+AGGACA)GATCCAGAAGACCACATGAGATTTCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613181042_bcik TTTACCTCCTGGAAATCTCATGTGGTCTTCTG(+AGGACA)GATCCGTCAAAAAAAAGTCAATTCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240613181040_rwcg GACACTCATATCCATTGGCACTGACAGCCTTG(+AGGACA)GTACCAGAAGGTTAGGTGAATGAACGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613181039_j1e6 AGGAAGACCCCGTTCATTCACCTAACCTTCTG(+AGGACA)GTACCAAGGCTGTCAGTGCCAATGGATATGA NGG 10 8 17 8 24 0 100 5 10