Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181112_xst5 ACTCATGTGACTGGTTTATTACAGTATGTAAG(+AGGACA)GATCCAAAGAAACTGATTCGTATCCACGCAG NGG 10 8 17 8 24 0 100 5 10
    240613181111_li84 GGGAACTGCGTGGATACGAATCAGTTTCTTTG(+AGGACA)GATCCTTACATACTGTAATAAACCAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240613181109_n3le TCTTACTAAAGTAATGGGCTCGTTTGCTATGG(+AGGACA)GATCCAGAACAACACTGTTTAGTATGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613181107_jy0r TTTGGAAGGACATACTAAACAGTGTTGTTCTG(+AGGACA)GATCCCATAGCAAACGAGCCCATTACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240613181105_jup8 TCGTTACCTCTAGAGGAAAGGCTGCTCGCCTG(+AGGACA)GTACCAAGACTACAGACAGGTGACAGAGCAA NGG 10 8 17 8 24 0 100 5 10
    240613181104_2ei3 TCTGATTGCTCTGTCACCTGTCTGTAGTCTTG(+AGGACA)GTACCAGGCGAGCAGCCTTTCCTCTAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613181102_965p GTCTATGACCCCATCCTTTAGGTCAGTTCTGG(+AGGACA)GATCCAATCTCTGGTCTTCACACTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613181101_rf5s TGCCTGCTGCAAAGTGTGAAGACCAGAGATTG(+AGGACA)GATCCCAGAACTGACCTAAAGGATGGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613181059_tcdx GTGCTCACCTGCATCAAACACAAAACACGATG(+AGGACA)GTACCTACTCTTTTCCCGAGTCCATGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240613181058_qmdq CTCCCGTAGCCATGGACTCGGGAAAAGAGTAG(+AGGACA)GTACCATCGTGTTTTGTGTTTGATGCAGGTG NGG 10 8 17 8 24 0 100 5 10