Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181129_g3d2 AATGATCCAAACCTTTAACATCGTTTCTGATG(+AGGACA)GTACCCAAGTTCACATTTTATTTTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240613181128_syjs TGATAAAAAATAAAAATAAAATGTGAACTTGG(+AGGACA)GTACCATCAGAAACGATGTTAAAGGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613181126_ckgr ATGTCTTATTCTAACCCTATGATCTTCGCTGG(+AGGACA)GATCCAGAGGGCCACGTGGCTCTGTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613181124_ptwv TGCTGTGCTCAACAGAGCCACGTGGCCCTCTG(+AGGACA)GATCCCAGCGAAGATCATAGGGTTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240613181122_f5wg GTGAAGAATAATCTATCTCTATTCCTGCCAGG(+AGGACA)GTACCTTATGTACTAAAGCGCCTTACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613181120_pytl TAATTCCTGAGTAAGGCGCTTTAGTACATAAG(+AGGACA)GTACCCTGGCAGGAATAGAGATAGATTATTC NGG 10 8 17 8 24 0 100 5 10
    240613181118_zzpx CATAGACCTCTCCTCTGCCACTGTATCTCGGG(+AGGACA)GTACCATTCAAGCACAGCCTGAGAGCATAAG NGG 10 8 17 8 24 0 100 5 10
    240613181117_o2b7 GTGACCTTATGCTCTCAGGCTGTGCTTGAATG(+AGGACA)GTACCCCGAGATACAGTGGCAGAGGAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613181115_8ttm TGCAGGAATCCTGCTACTCAGCCCTGCACTGG(+AGGACA)GTACCACCCCCAAGCCCCCATTCCTTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613181113_0gbd ATGCCAGGGGAAGGAATGGGGGCTTGGGGGTG(+AGGACA)GTACCCAGTGCAGGGCTGAGTAGCAGGATTC NGG 10 8 17 8 24 0 100 5 10