Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181233_9mym ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+AGGACA)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613181232_06c6 CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+AGGACA)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613181230_ff8j TATCCCGAGGACAGTTACTAAGCTATACTTGG(+AGGACA)GATCCCACTCAGAAGGTTTCAGAACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613181229_hn7m GAAGGACCCCAGTTCTGAAACCTTCTGAGTGG(+AGGACA)GATCCCAAGTATAGCTTAGTAACTGTCCTCG NGG 10 8 17 8 24 0 100 5 10
    240613181226_nabg CCATCCACACCCTGCAGCAAGTCCCAACCTGG(+AGGACA)GTACCACTGTTATTATTACAAGGAAAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613181225_jkh0 TTTAACTTTCTTTCCTTGTAATAATAACAGTG(+AGGACA)GTACCCAGGTTGGGACTTGCTGCAGGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613181224_zz1x AGAGAGGGATGTGGTGTGGACTCTGAGCTGAG(+AGGACA)GATCCTCTCCACGTTGTGATGGGGTAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613181222_7po7 CGGCCCCTCCTACCCCATCACAACGTGGAGAG(+AGGACA)GATCCTCAGCTCAGAGTCCACACCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240613181221_vnxx GGGAATCTGACACCTTCCGCTGGGTTCTGAGG(+AGGACA)GTACCTGTACTCATGTGTACCCTGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613181220_2qd2 GTGGGGAGGGGGCAGGGTACACATGAGTACAG(+AGGACA)GTACCCTCAGAACCCAGCGGAAGGTGTCAGA NGG 10 8 17 8 24 0 100 5 10