Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181201_hq65 AGATTTTGAGATCACATCGTTCTCCCCTCAAG(+AGGACA)GATCCCCTGATTAGTGGGAGACTAAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240613181159_yw4e GGGGGCAGGTCTTAGTCTCCCACTAATCAGGG(+AGGACA)GATCCTTGAGGGGAGAACGATGTGATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613181157_d2pg TGAGAGAAGGGCTGGGAGCCAGCCAAGGGATG(+AGGACA)GTACCTTTGTTGTGACGGGGCTACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613181156_3o5n GCTGAGAAGGGTGTAGCCCCGTCACAACAAAG(+AGGACA)GTACCATCCCTTGGCTGGCTCCCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613181155_hfb5 AGAAGTCCAGATACTCCATTCTTGAGTGTCAG(+AGGACA)GATCCCAGGCAAAACTGGCAATACAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613181153_70t7 GTTTCCTGGATTGTATTGCCAGTTTTGCCTGG(+AGGACA)GATCCTGACACTCAAGAATGGAGTATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613181151_wukp GTGCTTGCTGCCAGGTTTGACCACGTGAGTTG(+AGGACA)GATCCCTGGAACCCACATGGTGGAAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613181149_by7e CAATTCTCTCTTTCCACCATGTGGGTTCCAGG(+AGGACA)GATCCAACTCACGTGGTCAAACCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613181148_2tg8 GTGGTCATTGAGTGCCACCAGTACATCTGATG(+AGGACA)GATCCAGTTGGCACGTCATGACAGATTGACT NGG 10 8 17 8 24 0 100 5 10
    240613181147_22az ACACAAGTCAATCTGTCATGACGTGCCAACTG(+AGGACA)GATCCATCAGATGTACTGGTGGCACTCAATG NGG 10 8 17 8 24 0 100 5 10