Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181403_zs77 GTGCTAGCTGCTGCCACCCCTGAGCCGTCGTG(+AGGACA)GATCCCACTGTCGCGCACAGCACCTGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240613181402_8ier AGGGAGTCCACAGGTGCTGTGCGCGACAGTGG(+AGGACA)GATCCACGACGGCTCAGGGGTGGCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613181400_ipw1 CTAGGAGGTGCCCAGAGAAGGACAACGTGGGG(+AGGACA)GTACCTCAGAGTGGGTAGAGATGCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613181358_fsyo CCACAGCCTGTGGCATCTCTACCCACTCTGAG(+AGGACA)GTACCCCCACGTTGTCCTTCTCTGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240613181357_3dgp ATTGTGGGAATAAAGCCTCTGGCTGAGATATG(+AGGACA)GTACCCCATGGGCTTCTTGCAAGGGAATCCT NGG 10 8 17 8 24 0 100 5 10
    240613181355_zyyr ATTTGAGGATTCCCTTGCAAGAAGCCCATGGG(+AGGACA)GTACCATATCTCAGCCAGAGGCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10
    240613181352_cu5d TTGCCTTCCAGAAAGCCTCCATCTAGCCCCAG(+AGGACA)GTACCCCCAGGACTCCAGCCTTTAAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240613181350_s2mc TTGTTAGTTCTTTAAAGGCTGGAGTCCTGGGG(+AGGACA)GTACCTGGGGCTAGATGGAGGCTTTCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240613181348_iqjw GACCTCCGGGGCTTTGGCCTCCTGGGACCACG(+AGGACA)GTACCCAGGGCTCTCTGCCTTTAGGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613181345_ad9r ACAAGAAGCTGCCTAAAGGCAGAGAGCCCTGG(+AGGACA)GTACCGTGGTCCCAGGAGGCCAAAGCCCCGG NGG 10 8 17 8 24 0 100 5 10