Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181344_qua6 CTGTATATGTAGGTGCCCATGGAGGGATGTTG(+AGGACA)GATCCCCTGGAGCTAGAGTTTCATGAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613181341_qgrr CAACTTACACTCATGAAACTCTAGCTCCAGGG(+AGGACA)GATCCAACATCCCTCCATGGGCACCTACATA NGG 10 8 17 8 24 0 100 5 10
    240613181338_01dc GCCTAGTGCTACAGAGCCAGGCAGGAACCAGG(+AGGACA)GTACCAGCCTCTAGGCCTGGTGCCTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240613181337_bkvt AGAAGTGATGCAGGCACCAGGCCTAGAGGCTG(+AGGACA)GTACCCTGGTTCCTGCCTGGCTCTGTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613181335_jbwt ATGAAGTTGTGAGCAGCTGCTCTGTCTTTTGG(+AGGACA)GATCCTGTTACGATCTGACAAATTAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613181334_44ha TAAGGCAGACTTAATTTGTCAGATCGTAACAG(+AGGACA)GATCCCAAAAGACAGAGCAGCTGCTCACAAC NGG 10 8 17 8 24 0 100 5 10
    240613181332_gw84 GTTGACCTGGTGATATTATCCTGGAGTCTCTG(+AGGACA)GATCCAGCCACGCTTCATCTCCAACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240613181331_lsvu GGAAAGGATGAGTTGGAGATGAAGCGTGGCTG(+AGGACA)GATCCAGAGACTCCAGGATAATATCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240613181330_9nm9 GAGCTACAAACCATGTTTGTAAATGCTCAGCG(+AGGACA)GATCCTTTCAACACACTCTAAGATACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613181328_ewsh TGTTCTTTGAGTATCTTAGAGTGTGTTGAAAG(+AGGACA)GATCCGCTGAGCATTTACAAACATGGTTTGT NGG 10 8 17 8 24 0 100 5 10