Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181037_3gm3 ATGACAATGCAAGGTTCACCTCAGTGTTGAGG(+AGGACA)GATCCAAGCTGAGGTGAAGCTGGGGACTATG NGG 10 8 17 8 24 0 100 5 10
    240613181035_1fjl ACATCCATAGTCCCCAGCTTCACCTCAGCTTG(+AGGACA)GATCCCTCAACACTGAGGTGAACCTTGCATT NGG 10 8 17 8 24 0 100 5 10
    240613181033_h6gm TTTTAGGTTAGCTTCACTGTTGATATCCTTGG(+AGGACA)GTACCTGCCTTCCAAATGGTGGGATTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613181031_k05o ACAGGCCTACAATCCCACCATTTGGAAGGCAG(+AGGACA)GTACCCAAGGATATCAACAGTGAAGCTAACC NGG 10 8 17 8 24 0 100 5 10
    240613181028_wuzl CTATTCAGTAACCTCAGGCTCTGAGTCCCAGG(+AGGACA)GATCCTGAGAGATCACTGCACGGGCCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613181027_smuq GCTGCAGGAAGGCCCGTGCAGTGATCTCTCAG(+AGGACA)GATCCCTGGGACTCAGAGCCTGAGGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240613181025_r9sa CTTCTTGGAGTTTGAAAAATGTGTTGATAATG(+AGGACA)GATCCGCAGTAGCTTTCAGGGAGACGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613181024_3f46 GTCACTGACCCGTCTCCCTGAAAGCTACTGCG(+AGGACA)GATCCATTATCAACACATTTTTCAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613181023_x4t7 GAGCCAAGCTTCCAGGTAGAATGGAGAATGTG(+AGGACA)GATCCCAGTTGGACCCCTGGGCTTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613181021_7bya GGGCCAGGTGGAAAGCCCAGGGGTCCAACTGG(+AGGACA)GATCCACATTCTCCATTCTACCTGGAAGCTT NGG 10 8 17 8 24 0 100 5 10