Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181239_tsks AAATAAAATAAATAGAATAAAGTATTCAAAGG(+AGGACA)GTACCACCACTCCAATTGCATCAAGCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613181237_g9qx GTCCAAGGTGGCTTGATGCAATTGGAGTGGTG(+AGGACA)GTACCCTTTGAATACTTTATTCTATTTATTT NGG 10 8 17 8 24 0 100 5 10
    240613181236_cjm0 AAGTATTGTTTCCTAATCAGACTTTTACATTG(+AGGACA)GTACCATGAGTTCTTTATGCTTTGCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613181235_tb6x ACAATGTCTTGGCAAAGCATAAAGAACTCATG(+AGGACA)GTACCAATGTAAAAGTCTGATTAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240613181233_9mym ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+AGGACA)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613181232_06c6 CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+AGGACA)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613181230_ff8j TATCCCGAGGACAGTTACTAAGCTATACTTGG(+AGGACA)GATCCCACTCAGAAGGTTTCAGAACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613181229_hn7m GAAGGACCCCAGTTCTGAAACCTTCTGAGTGG(+AGGACA)GATCCCAAGTATAGCTTAGTAACTGTCCTCG NGG 10 8 17 8 24 0 100 5 10
    240613181226_nabg CCATCCACACCCTGCAGCAAGTCCCAACCTGG(+AGGACA)GTACCACTGTTATTATTACAAGGAAAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613181225_jkh0 TTTAACTTTCTTTCCTTGTAATAATAACAGTG(+AGGACA)GTACCCAGGTTGGGACTTGCTGCAGGGTGTG NGG 10 8 17 8 24 0 100 5 10