Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181224_zz1x AGAGAGGGATGTGGTGTGGACTCTGAGCTGAG(+AGGACA)GATCCTCTCCACGTTGTGATGGGGTAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613181222_7po7 CGGCCCCTCCTACCCCATCACAACGTGGAGAG(+AGGACA)GATCCTCAGCTCAGAGTCCACACCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240613181221_vnxx GGGAATCTGACACCTTCCGCTGGGTTCTGAGG(+AGGACA)GTACCTGTACTCATGTGTACCCTGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613181220_2qd2 GTGGGGAGGGGGCAGGGTACACATGAGTACAG(+AGGACA)GTACCCTCAGAACCCAGCGGAAGGTGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613181218_fbri TGAAAACACAAGGACCTGAGCTGGGATCCTTG(+AGGACA)GTACCCAGAGAAAAAAATCTGGGTCTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613181216_8tw6 ACGGCCCACCAGACCCAGATTTTTTTCTCTGG(+AGGACA)GTACCAAGGATCCCAGCTCAGGTCCTTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613181214_nphh GCTCGCTGTGAAAACACAAGGACCTGAGCTGG(+AGGACA)GATCCTTGGTACCCAGAGAAAAAAATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613181213_tyne CCAGACCCAGATTTTTTTCTCTGGGTACCAAG(+AGGACA)GATCCCAGCTCAGGTCCTTGTGTTTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240613181210_tn93 TAGCAAACCAAAAAACACCCCTTTTCCCAAGG(+AGGACA)GATCCTACATCTCTAGCATTTGTGAAACCTG NGG 10 8 17 8 24 0 100 5 10
    240613181209_ohet GACCCCAGGTTTCACAAATGCTAGAGATGTAG(+AGGACA)GATCCCTTGGGAAAAGGGGTGTTTTTTGGTT NGG 10 8 17 8 24 0 100 5 10