Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180811_y6bd TTTTGCCAATGAGTCTGAGGCTGTGGTCTCTG(+AGGACA)GATCCAGCCATGATGTTTTTATATGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613180810_uzv4 GTTTAATTAAACATATAAAAACATCATGGCTG(+AGGACA)GATCCAGAGACCACAGCCTCAGACTCATTGG NGG 10 8 17 8 24 0 100 5 10
    240613180808_cneo AGTTGTGAAAGCAAGGAGTACAGAATGCTAGG(+AGGACA)GATCCTCTCAAAAGTTCCAAACTAATTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613180807_9ue8 GGAACACAGAATTAGTTTGGAACTTTTGAGAG(+AGGACA)GATCCCTAGCATTCTGTACTCCTTGCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613180805_6ddf TTGCCCAGGCCCCTCCAGAAGTACTGTGTTTG(+AGGACA)GTACCAGCATTTGTTTGTAGTTTTTATATGT NGG 10 8 17 8 24 0 100 5 10
    240613180804_k7g3 CAATCACATATAAAAACTACAAACAAATGCTG(+AGGACA)GTACCAAACACAGTACTTCTGGAGGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613180803_2mmg ATCTATGAACATGTTTGGTTGGGATGTGTTTG(+AGGACA)GATCCAGGACCTGGGTCCCCTGGGAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240613180801_h7ae GCCCTAACATTTCCCAGGGGACCCAGGTCCTG(+AGGACA)GATCCAAACACATCCCAACCAAACATGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613180759_gai5 TAGACTCCCTCTGGGAGCTCTTGGTGGCTTGG(+AGGACA)GTACCCTGACCTGGCCTTGGGGACTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613180757_k5fs CAGTGCTCAGGAGTCCCCAAGGCCAGGTCAGG(+AGGACA)GTACCCAAGCCACCAAGAGCTCCCAGAGGGA NGG 10 8 17 8 24 0 100 5 10