Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180953_g8jn GCCTGCCGTGTGAGGCCCAACGCATAGAGCAG(+AGGACA)GTACCTGACTGCGTCCAGCCTTTCTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613180952_pui6 CCAAGCTGCCCAGAAAGGCTGGACGCAGTCAG(+AGGACA)GTACCTGCTCTATGCGTTGGGCCTCACACGG NGG 10 8 17 8 24 0 100 5 10
    240613180950_emsj CCGCTGGATGCATGGCCTGTGGGACCTCTGTG(+AGGACA)GTACCAGAGGTCCATTCCGTTCCTTCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613180948_i1fs GGGAGGCTGCGAAGGAACGGAATGGACCTCTG(+AGGACA)GTACCACAGAGGTCCCACAGGCCATGCATCC NGG 10 8 17 8 24 0 100 5 10
    240613180946_dcz2 CATTCCAGGTTCTAGAGCTTTCTATGGGGCGG(+AGGACA)GTACCTCGGTGCGCATAGGGAATGAGGACGA NGG 10 8 17 8 24 0 100 5 10
    240613180944_3nxi TAGCTTCGTCCTCATTCCCTATGCGCACCGAG(+AGGACA)GTACCCGCCCCATAGAAAGCTCTAGAACCTG NGG 10 8 17 8 24 0 100 5 10
    240613180943_g2k5 TCATGTTTCTCACTGGGGTCTTGGAACCTGTG(+AGGACA)GATCCCAGTCCCTATGTGATATGCTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240613180941_qf8f CAGGGCATTTGAGCATATCACATAGGGACTGG(+AGGACA)GATCCACAGGTTCCAAGACCCCAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613180939_x5at CATTCTACCCCATTATTTTAGTATTGGTATTG(+AGGACA)GATCCAATCAGGGCAAGAACATTGTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240613180938_nf1x TTTAATAACATACAATGTTCTTGCCCTGATTG(+AGGACA)GATCCAATACCAATACTAAAATAATGGGGTA NGG 10 8 17 8 24 0 100 5 10