Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160225_5lnz CAGGATGAGAACCAGGATCAGCCTGCGGGCCG(+AAAAAA)GTACCGGCCTGCCCCACTCTACAGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606160223_1r0r CCCACACCCTGGTGGCTAGAGGAGAGTAGGGG(+AAAAAA)GTACCCTACAGCCACTGGGGGCTCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160221_q3g4 GCCTGCAGGCAGGAGCCCCCAGTGGCTGTAGG(+AAAAAA)GTACCCCCTACTCTCCTCTAGCCACCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606160219_ax5b CTTGAGGCAGCCACCTCACCAGGAACACCTGG(+AAAAAA)GATCCAGCCCAGGCTGGGCCGATGTGAGGCG NGG 10 8 17 8 24 0 100 5 10
    240606160217_zx2m AGCCTCGCCTCACATCGGCCCAGCCTGGGCTG(+AAAAAA)GATCCCAGGTGTTCCTGGTGAGGTGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606160215_yqc7 CAGCTGCCTAAAGCTGGGCCTGGCTGGCCGGG(+AAAAAA)GATCCACTTAGTCCAGAAGGAGAAAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606160213_4zai ACCTTTGTCTCTTTCTCCTTCTGGACTAAGTG(+AAAAAA)GATCCCCGGCCAGCCAGGCCCAGCTTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606160212_17wh GTTCTGCTTGCCCAGGACAGCTCAGCCTGTAG(+AAAAAA)GATCCTCTCACAGGGAAGATGCTCTTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606160211_qhxe TGTTTTAGCCAAGAGCATCTTCCCTGTGAGAG(+AAAAAA)GATCCTACAGGCTGAGCTGTCCTGGGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606160209_e2n1 TCTAACATCTAGGTTGAGAACAAGCCTGAATG(+AAAAAA)GTACCCATGGTTGGGAGAGAGAACTTGAACT NGG 10 8 17 8 24 0 100 5 10