Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160134_ha40 CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+AAAAAA)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606160132_hed5 GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+AAAAAA)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606160131_vg0a GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+AAAAAA)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606160130_eiet AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+AAAAAA)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606160128_jclt CCCACCTGTCTGCCCATCATCACTAGGGGCGG(+AAAAAA)GTACCTACTGAAGAGGGAGGGCTGTTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606160127_qe5o AACCTGAAGACCAAAGAGAAGGCAGCCAAGTG(+AAAAAA)GATCCTGGCAGCTGTGGCGCTGCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606160125_es9p TACACCTGCAGGAGCAGCGCCACAGCTGCCAG(+AAAAAA)GATCCACTTGGCTGCCTTCTCTTTGGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606160124_hx2w GACAGACAGACAAGGCTGCACGCTCCCTCAAG(+AAAAAA)GATCCCGTCAGGACACTCCTGGCCTACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606160122_8p90 GAATTCAAAGTAGGCCAGGAGTGTCCTGACGG(+AAAAAA)GATCCTTGAGGGAGCGTGCAGCCTTGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606160121_4l77 ATTTGGGGGAGTTGGTTGTTTCCTTCCATATG(+AAAAAA)GATCCCCAAGGACCAAACACAGGTTGTCAGG NGG 10 8 17 8 24 0 100 5 10