Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160047_m9ym ATTGTAAATTGCTTAAAAAACATCCTGTGGTG(+AAAAAA)GATCCATGAAACAGCCAGGAAAGGCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606160046_4w9y TAAGATGGGGAACACATGGGGGAGGGGCTGAG(+AAAAAA)GATCCCTTTACCAAGGGCCTGAGGTCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160044_m5r9 TTGAATGTATGACCTCAGGCCCTTGGTAAAGG(+AAAAAA)GATCCTCAGCCCCTCCCCCATGTGTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606160042_bs2c CCCTGGTTGGGGTTCACCCGAGATGCCCAGGG(+AAAAAA)GTACCGCCTCCAGCCTCAGGCCTGGAGCATT NGG 10 8 17 8 24 0 100 5 10
    240606160040_t16v GATGGAATGCTCCAGGCCTGAGGCTGGAGGCG(+AAAAAA)GTACCCCTGGGCATCTCGGGTGAACCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240606160038_x524 ACAGAAGTCACTGTCTTGCTGTCTAACCCAAG(+AAAAAA)GATCCTATGTAGGGCCTCTCCAGAGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240606160036_w9m8 ATGCCTTGTCTCTCTGGAGAGGCCCTACATAG(+AAAAAA)GATCCTTGGGTTAGACAGCAAGACAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606160035_v32c CCTCTCCTGAGTCAGTTCTCTCTACCGTGTAG(+AAAAAA)GTACCAGGGATCTAACTCGTGGCAGTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606160033_djnd CGCTGTCCACACTGCCACGAGTTAGATCCCTG(+AAAAAA)GTACCTACACGGTAGAGAGAACTGACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606160031_cdsv GGACAAAAGGTTCCAAGTCGTGTGAACAGAAG(+AAAAAA)GATCCAGCTGCCAACTGGCATCCCAGTGCCA NGG 10 8 17 8 24 0 100 5 10