Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155945_kx8z CTGACATTCTGTGGGCTCATACCTATTTGGTG(+AAAAAA)GATCCTCAAGGTTTCTTCTGGATTGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606155944_uq9i GGAGGCAGGAACGCTTTGAAAAAGGCAAACGG(+AAAAAA)GATCCACTGAACCTCATCTTTATCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606155943_g0hh CCTCCACCCAAGGATAAAGATGAGGTTCAGTG(+AAAAAA)GATCCCGTTTGCCTTTTTCAAAGCGTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606155941_223k CACGGGGTGGGGGTGAGGGAAGCAGGCAGGTG(+AAAAAA)GATCCCTGAGGCTCACTGGACAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606155939_ez3h TAGGTCAGGCTGGCTGTCCAGTGAGCCTCAGG(+AAAAAA)GATCCACCTGCCTGCTTCCCTCACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606155938_fg45 CAGGAGGATCTCAAATTCAAGACCACTCTGGG(+AAAAAA)GTACCCAAGGAGACCCTGCCTCAAAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606155935_ep4i ATATTTGTGCTTTTGAGGCAGGGTCTCCTTGG(+AAAAAA)GTACCCCAGAGTGGTCTTGAATTTGAGATCC NGG 10 8 17 8 24 0 100 5 10
    240606155934_n9ei TTGAATAAATAGTTCAGGCTTTTCTCATCTCG(+AAAAAA)GTACCCAGGCTCCTGGAGACTGAAGATCAAC NGG 10 8 17 8 24 0 100 5 10
    240606155931_uhdi GGGTAGTTGATCTTCAGTCTCCAGGAGCCTGG(+AAAAAA)GTACCGAGATGAGAAAAGCCTGAACTATTTA NGG 10 8 17 8 24 0 100 5 10
    240606155930_nr8k CCCACACACACAATATTTTGCTACCTTTGTAG(+AAAAAA)GTACCCTTCCTAAGAAGCTGAAATGTATGAC NGG 10 8 17 8 24 0 100 5 10