Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160014_ov9a ATTTACACAGCATCCCTGAAGCAGCCTGCCTG(+AAAAAA)GTACCTTCCAAGGTACATTGGCTATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606160012_4am2 CCCGACCTATTTTGGTTTTGGGGGGCATACGG(+AAAAAA)GTACCTTTTGTGTTATTAGTTTATGTATATT NGG 10 8 17 8 24 0 100 5 10
    240606160011_spkz ATCTTAATATACATAAACTAATAACACAAAAG(+AAAAAA)GTACCCGTATGCCCCCCAAAACCAAAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606160009_mcnw TTTCAGTGGTATGTCAAGTACAAGGCCTCTGG(+AAAAAA)GATCCACACAAGCAGATGTTCTTGGAAATCA NGG 10 8 17 8 24 0 100 5 10
    240606160008_2elc ATGGATGATTTCCAAGAACATCTGCTTGTGTG(+AAAAAA)GATCCCAGAGGCCTTGTACTTGACATACCAC NGG 10 8 17 8 24 0 100 5 10
    240606160007_t8qu AGGCCCAGGATCTAGATTGCAGAGAACTTGAG(+AAAAAA)GATCCATGACTTAGCCAGCAGGTGTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606160006_4htk TGGAGCAAAACACACCTGCTGGCTAAGTCATG(+AAAAAA)GATCCTCAAGTTCTCTGCAATCTAGATCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160004_j200 GGCCCTGCTCAGATGGATGCAGAGCATTGAGG(+AAAAAA)GATCCAGATGAGGTGTGGTCCAGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606160003_j42i GATGCCCTCTTCCCTGGACCACACCTCATCTG(+AAAAAA)GATCCCTCAATGCTCTGCATCCATCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606160001_edk8 CCTAACCTTCCTTACCACACCAGCCTGGGACG(+AAAAAA)GATCCGAGCCCCAGCGGGAAGCCATTCTCTT NGG 10 8 17 8 24 0 100 5 10