Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160030_8mg8 CCAGGTGGCACTGGGATGCCAGTTGGCAGCTG(+AAAAAA)GATCCTTCTGTTCACACGACTTGGAACCTTT NGG 10 8 17 8 24 0 100 5 10
    240606160028_rhv8 TGCTTCCTACTTACTATAACAGAAGAGTTTGG(+AAAAAA)GTACCCCCTCCTCATGCACCCAAGAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606160026_w7gh GGGAACCTCCTTCTTGGGTGCATGAGGAGGGG(+AAAAAA)GTACCCAAACTCTTCTGTTATAGTAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606160025_ho4o ATGGTGCAGGAGAGGAGCTGAGAGTTCTACTG(+AAAAAA)GATCCTCAGAGAGTAGGAAGAGTGACACTAG NGG 10 8 17 8 24 0 100 5 10
    240606160023_g9yy CAGGCCTAGTGTCACTCTTCCTACTCTCTGAG(+AAAAAA)GATCCAGTAGAACTCTCAGCTCCTCTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606160022_qry1 GCGGATCTCCAGCCTGGAATCAGAAGTGTGAG(+AAAAAA)GTACCGGCAGCTCTGGTTCCTGCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606160020_4h19 AGAATCCAGTCCAGCAGGAACCAGAGCTGCCG(+AAAAAA)GTACCTCACACTTCTGATTCCAGGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606160019_b8sz GTGTGGGGGTGATGAGGGCAGCTTTTCAAGTG(+AAAAAA)GTACCCCGCAATTCAGGGGGTCCCTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606160017_mqkd CTTTGGAGCCCAGGGACCCCCTGAATTGCGGG(+AAAAAA)GTACCACTTGAAAAGCTGCCCTCATCACCCC NGG 10 8 17 8 24 0 100 5 10
    240606160016_lnir CATCCCCACTTATAGCCAATGTACCTTGGAAG(+AAAAAA)GTACCAGGCAGGCTGCTTCAGGGATGCTGTG NGG 10 8 17 8 24 0 100 5 10