Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155928_6dva ATGGGGTCATACATTTCAGCTTCTTAGGAAGG(+AAAAAA)GTACCTACAAAGGTAGCAAAATATTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606155927_p3wc GTACCCATGTACAATTATATTCACATTCTGAG(+AAAAAA)GTACCGGAGATTGGGGTCTCAGAGTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606155926_8ci2 CCCAGATTTGTACTCTGAGACCCCAATCTCCG(+AAAAAA)GTACCTCAGAATGTGAATATAATTGTACATG NGG 10 8 17 8 24 0 100 5 10
    240606155924_yitw TGCTCTGAGGACCCGTATGACGGGTACTCTGG(+AAAAAA)GTACCAGGCTACCAGGGACACACCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606155922_k46n AGAGCATCACTTGGTGTGTCCCTGGTAGCCTG(+AAAAAA)GTACCCAGAGTACCCGTCATACGGGTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606155921_s1jw GGTCACATCATCTTAACGTAAACATCAATAAG(+AAAAAA)GTACCAACATTGTTAATGGAGGAAAAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606155919_vylj TTTGGGTTGCTTTTCCTCCATTAACAATGTTG(+AAAAAA)GTACCTTATTGATGTTTACGTTAAGATGATG NGG 10 8 17 8 24 0 100 5 10
    240606155918_jo97 TGTCACGCAGAATGTGGTGTCCTGACTCAGTG(+AAAAAA)GATCCCCGTGCCTGCCGGGATCTGTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606155916_y32q AAGTGGTGTTCACAGATCCCGGCAGGCACGGG(+AAAAAA)GATCCACTGAGTCAGGACACCACATTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240606155914_j2pt TCTTTTCCTTAAGGTGACAGTACCATAGTAGG(+AAAAAA)GTACCCTTCTTTGCATGGTATGGAAGCATGA NGG 10 8 17 8 24 0 100 5 10