Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160150_qrmo TTGCTTGCTGCTTCCTGGTGGCCCTTGGTTAG(+AAAAAA)GATCCCTCTCTTTTCCTTCTGGAGCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240606160148_biha CAGGAGTCCCTGAGGTTCCCAGCTGCTGTCAG(+AAAAAA)GATCCTGTGAGTTGGGGCAAGCAGGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606160147_o2u8 GGTGGCAGCATCCTGCTTGCCCCAACTCACAG(+AAAAAA)GATCCTGACAGCAGCTGGGAACCTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606160145_m3ul CTGTCACATGCAGCAGACAGGCAGCATTAAGG(+AAAAAA)GTACCCTAGCTCTGTTAGGGCAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606160144_dw1y ACATTCCCAGGCTCTGCCCTAACAGAGCTAGG(+AAAAAA)GTACCCTTAATGCTGCCTGTCTGCTGCATGT NGG 10 8 17 8 24 0 100 5 10
    240606160142_rtb4 GGCGGCACTCGCCCCTCCTGGGGATACAGCAG(+AAAAAA)GATCCGGCCTCAAGAGGCTTCAGGTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240606160140_2iox GAGTGTCAAGCACCTGAAGCCTCTTGAGGCCG(+AAAAAA)GATCCTGCTGTATCCCCAGGAGGGGCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606160138_vue3 GGTGTAGCCCTACCTACAGTGTGACTAGGCCG(+AAAAAA)GTACCGATCCTGCCTGGTTCTAACCAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606160137_ui83 GTCTGTTTTCTGGTTAGAACCAGGCAGGATCG(+AAAAAA)GTACCGGCCTAGTCACACTGTAGGTAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606160136_i8ks AACCCCTTTCAACAGCCCTCCCTCTTCAGTAG(+AAAAAA)GTACCCGCCCCTAGTGATGATGGGCAGACAG NGG 10 8 17 8 24 0 100 5 10