Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155432_kq1h CTGGTACCTACAGAGGCCAGAAGAAGGTGTTG(+AAAAAA)GATCCCCTGAAACTCAGGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606155431_ahye CCAGAGGGGACCTAAGACTCTAGAGCTACATG(+AAAAAA)GATCCAGGAACACTCCTAGCTTTCTTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606155429_u9hx AAGGCCCTACAAGAAAGCTAGGAGTGTTCCTG(+AAAAAA)GATCCATGTAGCTCTAGAGTCTTAGGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606155427_dz0s GAGTATAAACTGTGGGGTCCTCAGTCCCACAG(+AAAAAA)GATCCCAAGCCACTTGGCATGCCCAGCGAGT NGG 10 8 17 8 24 0 100 5 10
    240606155425_xrae TCATGACTCGCTGGGCATGCCAAGTGGCTTGG(+AAAAAA)GATCCTGTGGGACTGAGGACCCCACAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606155424_6pli AGTGTCTGGGTTTGATGGTTGTTTATGGGATG(+AAAAAA)GATCCAGAGGCACTTATCTGGACCTGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606155422_8l8f GCCAAAGCAGCAGGTCCAGATAAGTGCCTCTG(+AAAAAA)GATCCATCCCATAAACAACCATCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606155420_tzqt GACTCGTGGCTAGTACTGCTTCCCCAGACTGG(+AAAAAA)GTACCTCCTACCCCCCCTCCCCCCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240606155419_ao1e GGGGTGTGTGTGGGGGGGAGGGGGGGTAGGAG(+AAAAAA)GTACCCAGTCTGGGGAAGCAGTACTAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606155418_4eqi GCTTAAGCAAGCCAAGCAAAAGGAAGAATAAG(+AAAAAA)GTACCCTCAAAGATGGTGCCCTAAGCCTCCA NGG 10 8 17 8 24 0 100 5 10