Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155416_gb2s CCACCTGGAGGCTTAGGGCACCATCTTTGAGG(+AAAAAA)GTACCTTATTCTTCCTTTTGCTTGGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606155414_qw7k TCCGGTCAGGCCGCTCAGGGACAGATGGATGG(+AAAAAA)GTACCTGGTGCTGTCTGCAGAACTCAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606155413_86zv GACTGGAGACTGAGTTCTGCAGACAGCACCAG(+AAAAAA)GTACCCATCCATCTGTCCCTGAGCGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606155411_d2uh GAATTGTTGTGACTTCCACGGATGTACCATGG(+AAAAAA)GATCCATGTGCGTTAATAGAAAGGATTATGT NGG 10 8 17 8 24 0 100 5 10
    240606155409_k1y5 ATTTTACATAATCCTTTCTATTAACGCACATG(+AAAAAA)GATCCCATGGTACATCCGTGGAAGTCACAAC NGG 10 8 17 8 24 0 100 5 10
    240606155408_c88h CTGAGGCCCCAGGGGACAGGCTCTATGTGTTG(+AAAAAA)GTACCCGTGAGGTGACCACCATCCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606155407_rk86 TAGGAAGCCCCTGGATGGTGGTCACCTCACGG(+AAAAAA)GTACCAACACATAGAGCCTGTCCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606155403_337g CCGCTGAATCTTCCCCAATCCCCCAGACCCGG(+AAAAAA)GATCCCCCACCATGTCCATCCCATAACATAA NGG 10 8 17 8 24 0 100 5 10
    240606155401_6eih AACTGTTATGTTATGGGATGGACATGGTGGGG(+AAAAAA)GATCCCGGGTCTGGGGGATTGGGGAAGATTC NGG 10 8 17 8 24 0 100 5 10
    240606155359_eydo CAAACTTTGTGGCTTAGAAGCCTGAAGAAGGG(+AAAAAA)GATCCCTTCCCTCCGAGAGCGCTTAACCCTG NGG 10 8 17 8 24 0 100 5 10