Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160551_jsin ACAGTCCGTGAGACTGGCTATGGCTAGCCTTG(+AAAAAA)GTACCAGTAACAAAACGGAACATAACGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606160550_nxl7 TTGTTTAACCGTTATGTTCCGTTTTGTTACTG(+AAAAAA)GTACCAAGGCTAGCCATAGCCAGTCTCACGG NGG 10 8 17 8 24 0 100 5 10
    240606160549_2232 GTTTTTAGGCTCAGCTACTCCTGGAAATACAG(+AAAAAA)GATCCTGGGTATACAGTTAAATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606160547_9olo GGCGGTGAGAAGCTATTTAACTGTATACCCAG(+AAAAAA)GATCCTGTATTTCCAGGAGTAGCTGAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606160545_ycl8 CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+AAAAAA)GATCCCTGCACTCCCCATGCTCCAAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160544_1w8v CTTGACAGGGCTTGGAGCATGGGGAGTGCAGG(+AAAAAA)GATCCAGGACCCCCATCCCCGGCCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240606160542_v86p CCGATACACCAGGTCATTCCTCTGCCCACTTG(+AAAAAA)GTACCTGGTGTACTAAAGGACTGCATGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606160541_yqdr CTGCAAAGACATGCAGTCCTTTAGTACACCAG(+AAAAAA)GTACCAAGTGGGCAGAGGAATGACCTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606160539_l01n CTTGACAGGGCCTGGCGCATGGGGAGTGCAGG(+AAAAAA)GATCCAGGACCCCCATCCCCAACCCGATACA NGG 10 8 17 8 24 0 100 5 10
    240606160537_qait CCTGGTGTATCGGGTTGGGGATGGGGGTCCTG(+AAAAAA)GATCCCTGCACTCCCCATGCGCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10