Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161009_gxdi CCCCTTTAATTTTTTCCTGTTTTGAGTACTGG(+AAAAAA)GTACCTAATTCTGCAACATAATGCAATATTT NGG 10 8 17 8 24 0 100 5 10
    240606161008_xolg TCCAGAAATATTGCATTATGTTGCAGAATTAG(+AAAAAA)GTACCCAGTACTCAAAACAGGAAAAAATTAA NGG 10 8 17 8 24 0 100 5 10
    240606161006_gu7y ACCACATGGTGGCTCACAACCATCTATAATGG(+AAAAAA)GATCCAATGCCCCCTTCTGGAGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606161005_sx11 GCTGTCTTCAGACACTCCAGAAGGGGGCATTG(+AAAAAA)GATCCCATTATAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606161004_xrch GCTGTCTTCAGACACATCAGAAGAGGGTATCG(+AAAAAA)GATCCCATTACAGATGTGGTTGCTGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606161002_3toz AATTCAACTCCCAGCAACCACATCTGTAATGG(+AAAAAA)GATCCGATACCCTCTTCTGATGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606161000_cbic TAGGTAGCCTTGGCTGGCCTGGAACTCACAGG(+AAAAAA)GATCCACCTGTCTCTGCCACCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606160959_osbm CAATTCCAGCACTTGGGTGGCAGAGACAGGTG(+AAAAAA)GATCCCTGTGAGTTCCAGGCCAGCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606160958_fjli CAGGCAGACAGAGTAATGGAGAAGGAGCTGAG(+AAAAAA)GATCCTACGTCTTGATCTGAAAGTGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606160956_k3aw TTTCTCCTGACCACTTTCAGATCAAGACGTAG(+AAAAAA)GATCCTCAGCTCCTTCTCCATTACTCTGTCT NGG 10 8 17 8 24 0 100 5 10