Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155138_fo3e AACATCTTGTTCCTATATTTCTTCTGTGTAAG(+AAAAAA)GTACCTGGAGGGGATTTAGTTCTACTGAACC NGG 10 8 17 8 24 0 100 5 10
    240606155136_q6sz GCTCACAACCATCAGTAACTCTCATTCCAGGG(+AAAAAA)GATCCGGCACCTTCTTTTCACCTCTTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240606155134_2roo CTGGTACACGAAGAGGTGAAAAGAAGGTGCCG(+AAAAAA)GATCCCCTGGAATGAGAGTTACTGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606155133_ovig TTTGAATGTGCACACCATGCGTGTGCCTGTTG(+AAAAAA)GATCCCCTGGAACTGGGCTTGCGGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606155130_y7cz GCTCACAACCATCCGCAAGCCCAGTTCCAGGG(+AAAAAA)GATCCAACAGGCACACGCATGGTGTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240606155129_ajbo AAGGGGCCCCCCACTCTACCCTGGGGAACTTG(+AAAAAA)GATCCTCTTCAGAACCCTACCATGTTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606155128_ixo1 ACACACAGACAACATGGTAGGGTTCTGAAGAG(+AAAAAA)GATCCAAGTTCCCCAGGGTAGAGTGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606155126_gh8w AATCCCAGCACTTAAGGGGGTGGAGGTAAGAG(+AAAAAA)GATCCTGAGTTCAAGGCCAGCCTGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606155125_fs43 TAATGTTAAGCCCAGGCTGGCCTTGAACTCAG(+AAAAAA)GATCCTCTTACCTCCACCCCCTTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606155123_wbcb GAGGCTAATACCAAACCTATAGCTACTCCTGG(+AAAAAA)GTACCCTGCCTCCAGGTGATCACAGCCATCC NGG 10 8 17 8 24 0 100 5 10