Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155104_pbmq GAGTGGCTGAGGGAGGGGGGAGGGCATCACTG(+AAAAAA)GATCCGCAGAGAAGTCAGATACTGTGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606155102_zad3 GCACATGAGCACTTCACGGGGTCTACCAGGTG(+AAAAAA)GTACCTGCAGCATCTTCCCTGTCTCTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606155101_45yh TCAGACTTGCAGAGACAGGGAAGATGCTGCAG(+AAAAAA)GTACCACCTGGTAGACCCCGTGAAGTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606155100_avow GGATGTTATACTGGCCTGGATTCTGCATAACG(+AAAAAA)GTACCACATGCTCCACCCCATCCTCCCTAGT NGG 10 8 17 8 24 0 100 5 10
    240606155059_9vxy GGAGAACTAGGGAGGATGGGGTGGAGCATGTG(+AAAAAA)GTACCGTTATGCAGAATCCAGGCCAGTATAA NGG 10 8 17 8 24 0 100 5 10
    240606155057_08pp AACAACCAGGCTGTTAATGGGCAAACATCTGG(+AAAAAA)GTACCTCGGCCGAGTCAGGATGATTCATCAA NGG 10 8 17 8 24 0 100 5 10
    240606155055_shk7 TTGCTTTGATGAATCATCCTGACTCGGCCGAG(+AAAAAA)GTACCCAGATGTTTGCCCATTAACAGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606155054_aq0z AACGGGTCAAAACCTTCAGACAGGAAGTCTGG(+AAAAAA)GTACCACTCTGCAAGGTTACAATCACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606155052_qr26 AGCAGGCAGAGTGATTGTAACCTTGCAGAGTG(+AAAAAA)GTACCCAGACTTCCTGTCTGAAGGTTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240606155051_32lz TAACCGAGGCTCCCCTCTTAGAGGAAGGCATG(+AAAAAA)GTACCGGCAGATTTTGGTAAGCAAAGGAATG NGG 10 8 17 8 24 0 100 5 10