Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155019_0vr6 TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+AAAAAA)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240606155018_sh64 CACCAGCTTGACCCTCTCGATCTACAGACATG(+AAAAAA)GATCCGATCATTGCACACATTATAGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606155016_l7cd TCTTGTGGAGCCTATAATGTGTGCAATGATCG(+AAAAAA)GATCCATGTCTGTAGATCGAGAGGGTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606155014_h5h8 GTCCCTCTGTCAGAATGAGCACACCTTACCTG(+AAAAAA)GATCCCAGATTTTCTGTACATGCGTTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606155013_b9ag ACAAAGAACAAACGCATGTACAGAAAATCTGG(+AAAAAA)GATCCAGGTAAGGTGTGCTCATTCTGACAGA NGG 10 8 17 8 24 0 100 5 10
    240606155011_93c0 CTACTGTGGCTGACATCCAAAGGTATGGGGAG(+AAAAAA)GATCCTTGCTAGGAAGACCTCAGTCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606155010_jgjl TAAGATAAAAGGACTGAGGTCTTCCTAGCAAG(+AAAAAA)GATCCTCCCCATACCTTTGGATGTCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606155009_vata GCACGCTTTTCCTCCTGCTCCAAGGAGCTGAG(+AAAAAA)GATCCACCATATCCTCACCCTGACAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606155007_r51m TGTGGCCTCCCTGTCAGGGTGAGGATATGGTG(+AAAAAA)GATCCTCAGCTCCTTGGAGCAGGAGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606155005_5bcn GATTCATGACTTCCTACAATGCCAGCTCCCAG(+AAAAAA)GATCCATTTATCTTTGCTGGAGATTACATAC NGG 10 8 17 8 24 0 100 5 10