Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154946_fa6w TCCTAAGTCTTCTTTCTTTTTATTTAGCCCAG(+AAAAAA)GATCCTAGCCCAGGAGATGGTGCTAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606154944_a8uk TCCTCAGAAAATTGGACATAGTACTACCAGAG(+AAAAAA)GATCCTGCAATACCTCTCCTGGGCATAGATC NGG 10 8 17 8 24 0 100 5 10
    240606154943_nbw4 TTCTGGATCTATGCCCAGGAGAGGTATTGCAG(+AAAAAA)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606154941_t9mm GGATGGACCATCTAGAGACTGCCATATCCACG(+AAAAAA)GATCCATCCCATAATTAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240606154939_d5pa GGTGTCATCGTTTGGAGGCTAATTATGGGATG(+AAAAAA)GATCCGTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606154938_dbyk CATCTTCATGAGTTGCTCTGTTATTTTGCAGG(+AAAAAA)GATCCTGGGCAGCTTTGGCATTATCATGCAC NGG 10 8 17 8 24 0 100 5 10
    240606154936_ojpa GGAGTGTGCATGATAATGCCAAAGCTGCCCAG(+AAAAAA)GATCCCTGCAAAATAACAGAGCAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240606154934_i2ym GCAACCCAATAATACTATCATAATCCGGACAG(+AAAAAA)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606154933_nb8g TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+AAAAAA)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240606154931_ksi5 TTGGTAGTTCATTCGAAAGTTGAGCTCATCCG(+AAAAAA)GTACCGGGCTGGTTTCCTATGAATGAGTTCA NGG 10 8 17 8 24 0 100 5 10