Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154857_7iap AGAAAGAAAGAAAGAAAGAGAGGAAGAAGAAG(+AAAAAA)GATCCCAAGGTTCCAGTTAGGAACTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606154856_afhx CTGATACTTGATGTTCCAGCCCAGCACCTCTG(+AAAAAA)GATCCTTGAAGTACCCATGATGGGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606154854_zc9c AAAGGCCTATTACCCATCATGGGTACTTCAAG(+AAAAAA)GATCCAGAGGTGCTGGGCTGGAACATCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606154853_5zr3 ACTCTTCCGAAGTTCTCACTCCTCCTGAACAG(+AAAAAA)GATCCCCTGACCCGTTAGGAGCCTGCTTCGT NGG 10 8 17 8 24 0 100 5 10
    240606154851_5395 TGTGCACGAAGCAGGCTCCTAACGGGTCAGGG(+AAAAAA)GATCCTGTTCAGGAGGAGTGAGAACTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240606154850_4c0w TCCTTGCTCACCCAAACTAGATATATATTTGG(+AAAAAA)GATCCATGGTTGTTCAGACTTTACATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606154848_h88r TAAGATCTTCATGTAAAGTCTGAACAACCATG(+AAAAAA)GATCCCAAATATATATCTAGTTTGGGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606154846_zbdm TTTGAATCCAATGATCCAGCTCCAAGGATTGG(+AAAAAA)GATCCAGTGAGCCCTCTCCAATCCAAACCTT NGG 10 8 17 8 24 0 100 5 10
    240606154845_j3ow TTATAAAGGTTTGGATTGGAGAGGGCTCACTG(+AAAAAA)GATCCCAATCCTTGGAGCTGGATCATTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606154844_pljs ATTGACTCTGAGGAGAGAAGGTTCCCAAGGAG(+AAAAAA)GATCCCATGTCCATTTTGCCCCCACCTTCAC NGG 10 8 17 8 24 0 100 5 10