Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606160238_ounw GTACGTAAGCAGAAACAGGATGTCAACTAGGG(+AAAAAA)GTACCAGGACCACAGCTCTGAACACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240606160235_hrql GGGAGTGAATCGGGGTGAGCAGGCTTTGCCCG(+AAAAAA)GATCCCAGAACTCTAGTCCGTAAGTCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606160234_7d9t TGGTTATGAGGACTTACGGACTAGAGTTCTGG(+AAAAAA)GATCCGGGCAAAGCCTGCTCACCCCGATTCA NGG 10 8 17 8 24 0 100 5 10
    240606160232_jbn2 CTTGCTGTGCAGGAGCCACATGGTTCTTCTGG(+AAAAAA)GTACCAGGGCCAGGCCTGGACACCACACAGT NGG 10 8 17 8 24 0 100 5 10
    240606160229_ppmj AACTAACTGTGTGGTGTCCAGGCCTGGCCCTG(+AAAAAA)GTACCCAGAAGAACCATGTGGCTCCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240606160227_miqs CATACTCCTGGGCTGTAGAGTGGGGCAGGCCG(+AAAAAA)GTACCGGCCCGCAGGCTGATCCTGGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606160225_5lnz CAGGATGAGAACCAGGATCAGCCTGCGGGCCG(+AAAAAA)GTACCGGCCTGCCCCACTCTACAGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606160223_1r0r CCCACACCCTGGTGGCTAGAGGAGAGTAGGGG(+AAAAAA)GTACCCTACAGCCACTGGGGGCTCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606160221_q3g4 GCCTGCAGGCAGGAGCCCCCAGTGGCTGTAGG(+AAAAAA)GTACCCCCTACTCTCCTCTAGCCACCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606160219_ax5b CTTGAGGCAGCCACCTCACCAGGAACACCTGG(+AAAAAA)GATCCAGCCCAGGCTGGGCCGATGTGAGGCG NGG 10 8 17 8 24 0 100 5 10