Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155049_tyaf AGCAGCATTCCTTTGCTTACCAAAATCTGCCG(+AAAAAA)GTACCATGCCTTCCTCTAAGAGGGGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606155048_x2w4 GGAAGCCAGGGCAGGGCAGAGCTCAGGTGATG(+AAAAAA)GTACCTTGTGACAAGCACAGGTTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606155047_ig97 GATGATCTGAAGAAACCTGTGCTTGTCACAAG(+AAAAAA)GTACCATCACCTGAGCTCTGCCCTGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606155045_no14 CAACCATTTCCCCTTCCTGGATCCTCTGTCTG(+AAAAAA)GTACCAAGCAATATATTTTTTTATTTTACCC NGG 10 8 17 8 24 0 100 5 10
    240606155044_8gmo TGCAAGGGTAAAATAAAAAAATATATTGCTTG(+AAAAAA)GTACCAGACAGAGGATCCAGGAAGGGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606155043_xf0o TACCAACCTTGCTAGCCTGTGGGGACTTTAAG(+AAAAAA)GTACCGTGATGCATTTCATAAGGCTAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606155041_j3b8 CATATTCCCCTAGCCTTATGAAATGCATCACG(+AAAAAA)GTACCTTAAAGTCCCCACAGGCTAGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606155040_1zzg ATTACCAACTGCTGTCTCTCTGCTGTGAAAAG(+AAAAAA)GTACCGTAATTCACGTGATCCATCTATACGT NGG 10 8 17 8 24 0 100 5 10
    240606155039_pez0 GTCACACGTATAGATGGATCACGTGAATTACG(+AAAAAA)GTACCTTTTCACAGCAGAGAGACAGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606155038_10xu GAGAACCTAACCTTAGTTTCAGGTCAAGGTTG(+AAAAAA)GTACCTCCTGGTGGAAATTCTCATTTGGATC NGG 10 8 17 8 24 0 100 5 10