Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155357_9g4e CATCTCAGGGTTAAGCGCTCTCGGAGGGAAGG(+AAAAAA)GATCCCCTTCTTCAGGCTTCTAAGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606155356_6zl0 GACACCCCTGCCTTAGCAAGGGGCTCCAGTTG(+AAAAAA)GATCCCGTTGAAGCACGGTGGCCGCGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606155354_0fhc GCCCTACCTTCGCGGCCACCGTGCTTCAACGG(+AAAAAA)GATCCAACTGGAGCCCCTTGCTAAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606155353_5jfy GGAACAGGGCAGGAGGAGAGAGAGAGTTTGTG(+AAAAAA)GATCCCCAGAAACTCTAGGCCCATATAGTCG NGG 10 8 17 8 24 0 100 5 10
    240606155351_296n TTGGTCGACTATATGGGCCTAGAGTTTCTGGG(+AAAAAA)GATCCACAAACTCTCTCTCTCCTCCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606155349_u17m ACCACATGATAGTTCACAACCATCTGCAATGG(+AAAAAA)GATCCAATGCCTTCTTCTGGTCTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606155348_icm3 ACTGTCTTCTGACAGACCAGAAGAAGGCATTG(+AAAAAA)GATCCCATTGCAGATGGTTGTGAACTATCAT NGG 10 8 17 8 24 0 100 5 10
    240606155347_b63x TGCTTCAAGGATAGGAGAGCCTCAGGTGAGAG(+AAAAAA)GTACCAACCACAGTGTGGGTGACTCGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606155345_eh0h CCAGAAGGGCCGAGTCACCCACACTGTGGTTG(+AAAAAA)GTACCTCTCACCTGAGGCTCTCCTATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606155344_e49s GGAAGGGATGATAAGAAACAGTCAAAGGGATG(+AAAAAA)GATCCCTTCTGCTGGGAAGAAACGGAAGGTC NGG 10 8 17 8 24 0 100 5 10