Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154739_prrk TGACTCCCCAGTGGAGGCAAGCCAGAGGTCTG(+AAAAAA)GATCCTCCACCCTCTCCTGGAGTGCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606154738_tfw4 CTTACTCTGGGGCACTCCAGGAGAGGGTGGAG(+AAAAAA)GATCCAGACCTCTGGCTTGCCTCCACTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606154736_scjn GGGGAGAAGGCCTGGCATCAAAAAGCTGCCAG(+AAAAAA)GATCCGCGACAAGGGGACGGACAAAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240606154735_2whh AGGGCTCTCATTTTGTCCGTCCCCTTGTCGCG(+AAAAAA)GATCCTGGCAGCTTTTTGATGCCAGGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606154733_6wal CAGCACAAGATGACTGGCCACTACCGGGGTGG(+AAAAAA)GTACCGCAGGGTTTGGGTTCAGGCTCTACAC NGG 10 8 17 8 24 0 100 5 10
    240606154731_yrsi CTGTTGTGTAGAGCCTGAACCCAAACCCTGCG(+AAAAAA)GTACCCACCCCGGTAGTGGCCAGTCATCTTG NGG 10 8 17 8 24 0 100 5 10
    240606154730_z69p ATTCATGCTGGCAATTGAGCGCATAATCGCTG(+AAAAAA)GTACCATGAACATGATATAACTTTTACTAAG NGG 10 8 17 8 24 0 100 5 10
    240606154729_8v77 TTAAACTTAGTAAAAGTTATATCATGTTCATG(+AAAAAA)GTACCAGCGATTATGCGCTCAATTGCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606154727_d8rb CAAAAGAAATACTCTTACACATTATAAGCCAG(+AAAAAA)GATCCACATAGGTTCAAATACCCCCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240606154726_qd68 GCTGACCACATGGGGGTATTTGAACCTATGTG(+AAAAAA)GATCCTGGCTTATAATGTGTAAGAGTATTTC NGG 10 8 17 8 24 0 100 5 10