Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154652_9orm TGAATACTAAAATTTCTTAAATGCAAGGTGTG(+AAAAAA)GATCCATTTTTCCCTCTCTGTGAATCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606154650_auu0 AAACCACTTCCCACCTCACAAGCAACTGGACG(+AAAAAA)GTACCTGTAGTTGGCAAAAGGCCACTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606154649_0ixm AGCAGTGGCCAGTGGCCTTTTGCCAACTACAG(+AAAAAA)GTACCGTCCAGTTGCTTGTGAGGTGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606154648_t06q GCAGAGCCCACCCCCCAGCCATGCTCAGCCTG(+AAAAAA)GATCCCTCCTTCCCAGAGCCTGTCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606154645_871e AGATCTCCCAGGGACAGGCTCTGGGAAGGAGG(+AAAAAA)GATCCAGGCTGAGCATGGCTGGGGGGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606154644_9wwg AAGACACAAAATTCTCCTGGTTCGCATGCACG(+AAAAAA)GTACCACTGTAAAAGCAACAAGAATAAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606154643_87ta AAAACCCCTTTATTCTTGTTGCTTTTACAGTG(+AAAAAA)GTACCGTGCATGCGAACCAGGAGAATTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606154642_6fxb GGAGGAGAGGTGGCGCCCAGCAGGTGGGTGAG(+AAAAAA)GATCCCCAGTGGTTGGCAGGGTGCCTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606154640_ux70 CCGCAAAGCCAGGCACCCTGCCAACCACTGGG(+AAAAAA)GATCCTCACCCACCTGCTGGGCGCCACCTCT NGG 10 8 17 8 24 0 100 5 10
    240606154638_nza6 CATATATGCCTGAAAGCCAGAAGAGGGCACCG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10