Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155817_tfu4 AGGAAGGGCCGGGGGACACTGGGCCAATTGGG(+AAAAAA)GATCCCCACGCACTCTGTTTGGTGTCGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606155815_e9f7 AGGCCCCTCACAACCCTTCTGCTCCAAGAGGG(+AAAAAA)GATCCAGCAGGTGCCTCTGCCCAGGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606155814_u9zw GGAAGCCAGGCCCTGGGCAGAGGCACCTGCTG(+AAAAAA)GATCCCCTCTTGGAGCAGAAGGGTTGTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606155812_fyb5 AACCTGATCAGAGCCTCCTTTCCTTGTTTTGG(+AAAAAA)GATCCTGAATGGGCAAGAGTCTGTCCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606155811_wy2y GGCCCACAGGGGACAGACTCTTGCCCATTCAG(+AAAAAA)GATCCCAAAACAAGGAAAGGAGGCTCTGATC NGG 10 8 17 8 24 0 100 5 10
    240606155809_8ktp GTCTACATCCTGGCTGGCTGGATGCCTACCTG(+AAAAAA)GTACCCACTTGCCATGCAGAGGCCTGTCCGG NGG 10 8 17 8 24 0 100 5 10
    240606155807_3hz1 ACCTCCCGGACAGGCCTCTGCATGGCAAGTGG(+AAAAAA)GTACCAGGTAGGCATCCAGCCAGCCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606155806_i38i GAGCAGCTTCTAGCCTGGGTCACGAAGAGCAG(+AAAAAA)GTACCCCTAGGAAGGCAGGAGAGTGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240606155804_7n7q GAACCCCTAGACACTCTCCTGCCTTCCTAGGG(+AAAAAA)GTACCTGCTCTTCGTGACCCAGGCTAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606155802_7jwf CTAACAACTGTCTGCAACTCCAGTCCCAAGGG(+AAAAAA)GATCCAAAGGTCTCTTCTGGCCTCTGCACAT NGG 10 8 17 8 24 0 100 5 10