Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154457_ljz2 TGCTGGGTGGAAGGTATGGGCGGGACTTCCAG(+AAAAAA)GTACCAGGAGGTAAAGGAAACACAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606154455_fccv GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+AAAAAA)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606154453_ah2h GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+AAAAAA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606154451_lhc1 TTCTCCATGGTGTGAGTGGCTCGTCTTGTAGG(+AAAAAA)GATCCTTCATGTGTCTCACCAACTGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606154450_04y6 GAAAAGGCAGCCAGTTGGTGAGACACATGAAG(+AAAAAA)GATCCCTACAAGACGAGCCACTCACACCATG NGG 10 8 17 8 24 0 100 5 10
    240606154447_x8o9 ATTGGCTGGATGGGATTGCCCTATTCCCCAGG(+AAAAAA)GATCCTCCTATTTCTGCTCCCCATTGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240606154445_bt1v ATAATCCTAGCAATGGGGAGCAGAAATAGGAG(+AAAAAA)GATCCCTGGGGAATAGGGCAATCCCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240606154444_24dj AAAAGACCTCAGGCACCTGAGCACTTTGACAG(+AAAAAA)GTACCAGGTAGAGTCCATCCTTGCATCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606154442_cv4f AGGAACACTGATGCAAGGATGGACTCTACCTG(+AAAAAA)GTACCTGTCAAAGTGCTCAGGTGCCTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606154440_ieq1 GCAGGTCCCACGGTGTTTGGAGATGGCTTGGG(+AAAAAA)GATCCGTGAGGATGGCATGACCAGCTAGCCC NGG 10 8 17 8 24 0 100 5 10