Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154405_tgwe CCCGGCTGAAGGCACAAACGCTGTGGTTAGGG(+AAAAAA)GTACCAAGGCAAGGCATGGATTCATTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606154404_mrev TGCTTTGTTTGAATAAACAGTATATCTTTGTG(+AAAAAA)GATCCTACTTTCTTTGACTGTAAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240606154403_3mis TTACAAGAATCCTTTACAGTCAAAGAAAGTAG(+AAAAAA)GATCCACAAAGATATACTGTTTATTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606154401_fjav CAAGAACAACTTAGAAGGTGGGCAGAAGGCAG(+AAAAAA)GTACCTCAGCTCCTTCAGCTTCTTTTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606154400_jcz5 AGATTGAGGAAAAAGAAGCTGAAGGAGCTGAG(+AAAAAA)GTACCTGCCTTCTGCCCACCTTCTAAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606154359_fan1 CCGAGCATAATCTCTAGGAAGGTTTTCTTTAG(+AAAAAA)GATCCCAAGGAGATGTCCGGAAACTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606154357_jlf3 GAGGCCTCAAGAGTTTCCGGACATCTCCTTGG(+AAAAAA)GATCCTAAAGAAAACCTTCCTAGAGATTATG NGG 10 8 17 8 24 0 100 5 10
    240606154354_6ono TGCAGCTGGGGGTGGCAAAGTGCAGAGCCCAG(+AAAAAA)GTACCAGGCCCCTGAGCTCCAGCAGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606154353_fswv TCTGCCTCTCCCTGCTGGAGCTCAGGGGCCTG(+AAAAAA)GTACCTGGGCTCTGCACTTTGCCACCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606154350_i04y CTTCCTCCAAGGACAGGCCTGTGAGTTCCGGG(+AAAAAA)GTACCTGTTACCTATTCCAGACATCTGAGTA NGG 10 8 17 8 24 0 100 5 10