Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154318_57dj CAAGAAAAAAATACAAAAGAAAGAAATACAAG(+AAAAAA)GATCCCTCAATATTCGTCTTATAAGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606154317_o075 TAGGGAGAGGCACTGAGAAGATGACAGGGCAG(+AAAAAA)GATCCAAGCCCTTCAACCAGAGCAAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240606154316_nczi TTTCTCCCAGTTTGCTCTGGTTGAAGGGCTTG(+AAAAAA)GATCCTGCCCTGTCATCTTCTCAGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606154314_3y5p GGAAGCCTTCCATGAAACAAATCTTGCAGCAG(+AAAAAA)GATCCCACCCAAGCTGGGTGGAGTTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606154313_r42y ACAGGTCCTTCAACTCCACCCAGCTTGGGTGG(+AAAAAA)GATCCTGCTGCAAGATTTGTTTCATGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606154311_1dmy GTTGAACTCAAGTACAACAGACCATACAATTG(+AAAAAA)GTACCATTCAAAATAATGACACTCCTTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606154310_7rbo AGGCAAAGAAAGGAGTGTCATTATTTTGAATG(+AAAAAA)GTACCAATTGTATGGTCTGTTGTACTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240606154309_u0y0 CTGGCAGAGAAATTAGAACTACCTTTTTCTAG(+AAAAAA)GATCCTCAGAAGCCAAACCACTTTGGTTATA NGG 10 8 17 8 24 0 100 5 10
    240606154308_3hfw GAGCATATAACCAAAGTGGTTTGGCTTCTGAG(+AAAAAA)GATCCTAGAAAAAGGTAGTTCTAATTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606154306_lltk TGGTGAGTAAGCCAGCGTCGGCTCAGAGCCTG(+AAAAAA)GATCCCAGTTACTTCCACATCTGCGGGGCCA NGG 10 8 17 8 24 0 100 5 10