Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154333_k2pm GGTAAGGGGTGGGGACCCAGGGCTGAATTGTG(+AAAAAA)GATCCCAGGGAAAGTGCTATGCACAGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606154331_s4c9 ATTATCCAACTCCCACCCCAGGATCTCCACTG(+AAAAAA)GTACCCAGATGCCCTTAGACCCTTTCATCTG NGG 10 8 17 8 24 0 100 5 10
    240606154330_cr7l GCTTCCAGATGAAAGGGTCTAAGGGCATCTGG(+AAAAAA)GTACCAGTGGAGATCCTGGGGTGGGAGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606154328_1mdb ACCTCAAGGCTTGGGTGACACACACAAATGTG(+AAAAAA)GTACCTCAGCCAGGGCCAGGACCTGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606154327_28f9 TGGACCCCTAGCAGGTCCTGGCCCTGGCTGAG(+AAAAAA)GTACCACATTTGTGTGTGTCACCCAAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606154325_3j9k TTCTACAGGCTTAGGGGCCTTTCCAGGGATGG(+AAAAAA)GTACCACCAAGGACTAACCACTTTACTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606154324_z6xh GGGGAACAAAGTAAAGTGGTTAGTCCTTGGTG(+AAAAAA)GTACCCATCCCTGGAAAGGCCCCTAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606154323_hvqr TCTAATAGGTCATCCTGTGGCTTCCAGAGTAG(+AAAAAA)GTACCGCCAGCCCAGCTCAAGTTCTCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240606154321_p6qw CCGTTAAGCAGAGAACTTGAGCTGGGCTGGCG(+AAAAAA)GTACCTACTCTGGAAGCCACAGGATGACCTA NGG 10 8 17 8 24 0 100 5 10
    240606154320_oae8 TTCTTTTGTTTCTTATAAGACGAATATTGAGG(+AAAAAA)GATCCTTGTATTTCTTTCTTTTGTATTTTTT NGG 10 8 17 8 24 0 100 5 10