Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155705_iuxf GCACATTGTCCTCCAGCCAATAAGAAAAGTGG(+AAAAAA)GATCCTCCCACCCCATCCTAACTTTCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606155703_xlz3 TCTTACTCCTGAGTATCCCAGGAGCTCAAAGG(+AAAAAA)GTACCGGGTGGTGTGCAGCGGGGTTAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606155701_iydf TGGCCCCTTCTAACCCCGCTGCACACCACCCG(+AAAAAA)GTACCCTTTGAGCTCCTGGGATACTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606155659_v5h6 GCTTCAGTGGAGGCCGGCCGGGGGAGGGAAGG(+AAAAAA)GATCCTATATTTCCTGCTGACTCTTATTAGG NGG 10 8 17 8 24 0 100 5 10
    240606155658_c9lo CTGACCCTAATAAGAGTCAGCAGGAAATATAG(+AAAAAA)GATCCCTTCCCTCCCCCGGCCGGCCTCCACT NGG 10 8 17 8 24 0 100 5 10
    240606155656_f8kx TGTGCACTGCCAGGGAGGTAGGTGTTCATGGG(+AAAAAA)GTACCTCTGTACCCAGGTGCTGCCCACTCCC NGG 10 8 17 8 24 0 100 5 10
    240606155655_yot1 ACACGGGGAGTGGGCAGCACCTGGGTACAGAG(+AAAAAA)GTACCCCATGAACACCTACCTCCCTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606155653_iu8i CTTTAGTGGCACTTGCCACACAGCTCCAGGGG(+AAAAAA)GTACCATTGGCACTGCAGGCTGGGCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606155650_8n1g TGCCACAGACTGCCCAGCCTGCAGTGCCAATG(+AAAAAA)GTACCCCCTGGAGCTGTGTGGCAAGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606155648_7u27 GTATAGAGAGAGTTGTTGTCGTGGGGCACCCG(+AAAAAA)GATCCCAGCAGCCTGGTTGCCTGCCTGTAGG NGG 10 8 17 8 24 0 100 5 10