Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154439_wwb9 TCTCGGGGCTAGCTGGTCATGCCATCCTCACG(+AAAAAA)GATCCCCAAGCCATCTCCAAACACCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606154437_xwt2 CCCCTTTCATGGTCCGTTTTCTTTTCAAACTG(+AAAAAA)GATCCAAAAAGAGACGAGGGTTTTCTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606154436_8yar CCTAGAGGAAAGAAAACCCTCGTCTCTTTTTG(+AAAAAA)GATCCAGTTTGAAAAGAAAACGGACCATGAA NGG 10 8 17 8 24 0 100 5 10
    240606154435_xmyk CTGGTAAAACTACTGCAGTGGGGCTTTGACAG(+AAAAAA)GATCCAAGTTATCACCAGAAAACAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240606154434_c6bh CACTTTTTGTTTTGTTTTCTGGTGATAACTTG(+AAAAAA)GATCCTGTCAAAGCCCCACTGCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606154432_iedj CCAAATACAGAAAAGAGGAGGCCTGAAAGAGG(+AAAAAA)GTACCAGGGGACAGGGAGCCATGGGGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606154429_48bt AACCCTAGGCCCCCATGGCTCCCTGTCCCCTG(+AAAAAA)GTACCCTCTTTCAGGCCTCCTCTTTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606154428_q3v8 ATTCCCAGTCAGCTTCAGGGGAATGAGAAAGG(+AAAAAA)GATCCTACTAATGTGGAAAGAAGCAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606154426_g58m GGAGGGATGCTTGCTTCTTTCCACATTAGTAG(+AAAAAA)GATCCCTTTCTCATTCCCCTGAAGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240606154425_247l CCTTGCTGTTAATTTTGCATATGCTAAAGCGG(+AAAAAA)GATCCCCTGGAGGCGGCATTTTCCCTTAGCT NGG 10 8 17 8 24 0 100 5 10