Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606155526_80az AATCAATTGGTTGAGGTGGCGAAGGTCAGCAG(+AAAAAA)GATCCAGGCATATCATTGTTTCATTCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606155525_sc2h GGTGTCTTTATCAAAGGTATATATAGAATTTG(+AAAAAA)GTACCTCTCAGGTTGTATCTCTAAAGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606155524_xzu6 TTCCTGTAACCTTTAGAGATACAACCTGAGAG(+AAAAAA)GTACCAAATTCTATATATACCTTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606155521_a4ka CCTACACATTAAATGTTTCTTTCAGGCTGGGG(+AAAAAA)GTACCGCAAGGTGGCAGAACTTCACCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606155520_o21e CTATGTGCTAGGTGAAGTTCTGCCACCTTGCG(+AAAAAA)GTACCCCCAGCCTGAAAGAAACATTTAATGT NGG 10 8 17 8 24 0 100 5 10
    240606155519_li0t GTTCCTTTTCTCGGTTTGCTGAGGGCCTCTTG(+AAAAAA)GATCCACTGGCCCAGAGATTTAAAAGTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606155517_44f6 CAACCCCAAACTTTTAAATCTCTGGGCCAGTG(+AAAAAA)GATCCAAGAGGCCCTCAGCAAACCGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606155516_eu4c AGTATATCTCTGCCCCAGCTCCAGTCTTGCTG(+AAAAAA)GATCCTCCTCCAGCTCCAACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606155515_6shf GTGTGTGTGTGTGTGTGTTGGAGCTGGAGGAG(+AAAAAA)GATCCAGCAAGACTGGAGCTGGGGCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606155513_tl96 CGACTCGGCGGTGAACGAACCTCAGGGTGCGG(+AAAAAA)GATCCTACACACACTCCCTTTCCCCACCCCC NGG 10 8 17 8 24 0 100 5 10