Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154622_azq3 CTTCCAAGGGATGCACACCCAGCTGCTTCATG(+AAAAAA)GTACCCGCAGGATGCAAGACAGTTTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240606154621_tgqu GAGTGGAGGTGCCTGCAGGGTCAGAGGTATTG(+AAAAAA)GATCCCTCTGGAGCTGGAGTTACAGTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606154619_yofd CTTACAACTGACTGTAACTCCAGCTCCAGAGG(+AAAAAA)GATCCAATACCTCTGACCCTGCAGGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240606154617_92cf CCTGCCTCATGGCCTTGCTCTGTAACTACCTG(+AAAAAA)GTACCCCTTCTAGACTTCCTTTAGGCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606154615_f0s1 AAAGGAGGCTGCCTAAAGGAAGTCTAGAAGGG(+AAAAAA)GTACCAGGTAGTTACAGAGCAAGGCCATGAG NGG 10 8 17 8 24 0 100 5 10
    240606154613_xwjy GGTGGCAGCCTCAGGATCTTGCCTTAGTCCTG(+AAAAAA)GTACCCAGATCTGTTCTCCTGAGACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240606154611_2v74 CTCTTGGATGAGTCTCAGGAGAACAGATCTGG(+AAAAAA)GTACCAGGACTAAGGCAAGATCCTGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606154609_iu0i CCAGCCTCCCATAAGGGTCAATACAACGCCTG(+AAAAAA)GATCCCCAAACCTGTGCTCAGGAGGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606154607_v9aa AGCCCCAGCTTCCTCCTGAGCACAGGTTTGGG(+AAAAAA)GATCCAGGCGTTGTATTGACCCTTATGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606154605_uqq5 CCCTGATGGAGGAGGCCAGGGCTCAGAGCAGG(+AAAAAA)GTACCCACTGTGGATCTTTGTCTGTGAACCG NGG 10 8 17 8 24 0 100 5 10