Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153209_c5ts TGAGGCCTGGCATTATTAGAGCATCTCACAGG(+AAAAAA)GATCCTTTCTACATTAGATCAGAAGCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606153208_wniw GGACCCCTGGGCTTCTGATCTAATGTAGAAAG(+AAAAAA)GATCCCTGTGAGATGCTCTAATAATGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606153206_zuzh CTGCGAACCTACTCAGGGCTATTTGCTCTCTG(+AAAAAA)GATCCCTGGTCTGCATTCACTCCATAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606153204_03hf CCCACATTTTTATGGAGTGAATGCAGACCAGG(+AAAAAA)GATCCAGAGAGCAAATAGCCCTGAGTAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606153202_ek7d CTACTGTAATCCCAGAAATTGGAATTCCTTGG(+AAAAAA)GTACCCAAGTCCCCTGCTATAATTAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606153200_7p5w ATTGTGCCTACTAATTATAGCAGGGGACTTGG(+AAAAAA)GTACCCAAGGAATTCCAATTTCTGGGATTAC NGG 10 8 17 8 24 0 100 5 10
    240606153159_36mr TGGACAAAGCGGGATGTGCGCAGAATCTAATG(+AAAAAA)GATCCCGGCAAATCTTCCAGGTAGGAATATT NGG 10 8 17 8 24 0 100 5 10
    240606153156_7swu GGAACAATATTCCTACCTGGAAGATTTGCCGG(+AAAAAA)GATCCATTAGATTCTGCGCACATCCCGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606153154_44ez AGGACTAGGGCAGAAGTCAAAAGCCTTCCTGG(+AAAAAA)GTACCTGCAGACAAGCGGGCAAGGGTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606153153_pxod TGCCAATCTCACCCTTGCCCGCTTGTCTGCAG(+AAAAAA)GTACCCAGGAAGGCTTTTGACTTCTGCCCTA NGG 10 8 17 8 24 0 100 5 10