Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153151_fmce AAGTGTGGAGGGGGGTGTACCCCTGAACACGG(+AAAAAA)GATCCTGTGTGCAGCCAAATGAGTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606153150_49e7 TGCCAGCCTGCAACTCATTTGGCTGCACACAG(+AAAAAA)GATCCCGTGTTCAGGGGTACACCCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606153149_021x AGAAGATGGAACCCTCACATTTGAGGGGCTTG(+AAAAAA)GTACCTCAACTAGATAGCACCGGGGACCAAG NGG 10 8 17 8 24 0 100 5 10
    240606153147_cpip TGATGCTTGGTCCCCGGTGCTATCTAGTTGAG(+AAAAAA)GTACCAAGCCCCTCAAATGTGAGGGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606153146_ajov GAGAGAGTTGGGCAGGGGTGGGGTGCAGAAAG(+AAAAAA)GATCCCCTTGCACAAAATCTTTGGCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606153144_m23f TTACTTGCAGTGCCAAAGATTTTGTGCAAGGG(+AAAAAA)GATCCTTTCTGCACCCCACCCCTGCCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606153143_xan6 ATCAGGCTATAAGCTTTGTAACTGCTACTAAG(+AAAAAA)GATCCAGGGTACACCCTGTCCTGCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606153140_ocrh ACAGCTGTCTCTGCAGGACAGGGTGTACCCTG(+AAAAAA)GATCCTTAGTAGCAGTTACAAAGCTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240606153139_4hny AAAATGTACAAAGTCTGAATAAAGCATTTTGG(+AAAAAA)GATCCGCCATCCGGTCTCTGCATATACAGAT NGG 10 8 17 8 24 0 100 5 10
    240606153137_dhz2 GTGTCATCTGTATATGCAGAGACCGGATGGCG(+AAAAAA)GATCCCAAAATGCTTTATTCAGACTTTGTAC NGG 10 8 17 8 24 0 100 5 10