Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153048_9bsm AATGAGCTACCCAACTTCTACCACATTCCTGG(+AAAAAA)GTACCCAACTGTGTTGTTACTCTGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240606153046_f7u5 AAACTGTGGGTTCAGAGTAACAACACAGTTGG(+AAAAAA)GTACCCAGGAATGTGGTAGAAGTTGGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606153045_2xmv GAAGAAGCCCAAGAAAAAAGCAGGGTTATCTG(+AAAAAA)GTACCTATCCTGGGCAGAATCACAGGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606153044_5r9z ATCAGTACAACCTGTGATTCTGCCCAGGATAG(+AAAAAA)GTACCAGATAACCCTGCTTTTTTCTTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606153043_mj33 CACACGTTAAGATGCCTTTCTCGCCTCTGAAG(+AAAAAA)GATCCTGAGTCACTGCCCTCATTTACTATGA NGG 10 8 17 8 24 0 100 5 10
    240606153041_3r13 GGGATTCATAGTAAATGAGGGCAGTGACTCAG(+AAAAAA)GATCCTTCAGAGGCGAGAAAGGCATCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606153040_0vwc TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+AAAAAA)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606153038_1n20 TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+AAAAAA)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606153037_t95v GTATTGTTTGTCAGCTCAGAAAGAATCAGGTG(+AAAAAA)GTACCCAAGTGCGGGGTCTTCCTAACGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606153035_x3t8 ATTTTTCAACGTTAGGAAGACCCCGCACTTGG(+AAAAAA)GTACCACCTGATTCTTTCTGAGCTGACAAAC NGG 10 8 17 8 24 0 100 5 10