Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153121_nhnb AGCATTTTGTCTGCATGTAATGGTACAGTCAG(+AAAAAA)GTACCATTACAACTGGCTTATTAAATTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606153120_zu7i TCTACATTGAATTTAATAAGCCAGTTGTAATG(+AAAAAA)GTACCTGACTGTACCATTACATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240606153118_uty9 AAAAAAAGTTTCAGAAAAATGATTTTATCTTG(+AAAAAA)GATCCATTTGTGAATACCTAGTGCAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240606153117_l10h CCCTGATAAGCTGCACTAGGTATTCACAAATG(+AAAAAA)GATCCAAGATAAAATCATTTTTCTGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606153115_0nqd ACTGTGGAATCTTCCTAGTTGTGCCTTCCAAG(+AAAAAA)GATCCTTTCATCATGTGGGTGAGCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240606153114_03r7 TGTAATGCATATGCTCACCCACATGATGAAAG(+AAAAAA)GATCCTTGGAAGGCACAACTAGGAAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240606153112_ega6 ACTGTGGAATCTTCCTAGTTGTGCCTTCCAAG(+AAAAAA)GATCCTTTCATCATGTGGGTGAGCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240606153110_552o TGTAATGCATATGCTCACCCACATGATGAAAG(+AAAAAA)GATCCTTGGAAGGCACAACTAGGAAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240606153108_caia GTATGGAAGGTTATATCCTACATACCCCAGCG(+AAAAAA)GTACCCCTTGACAGTTTACCATGCATAGACA NGG 10 8 17 8 24 0 100 5 10
    240606153106_vbzk TTACATGTCTATGCATGGTAAACTGTCAAGGG(+AAAAAA)GTACCGCTGGGGTATGTAGGATATAACCTTC NGG 10 8 17 8 24 0 100 5 10