Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606154226_0m6b TACTTGTAATACAAGCCTGGTGACCTGAGTAG(+AAAAAA)GATCCCCAAAACCTACTTAAAGATGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606154224_hwza GTCAGAGCTGCTACATGGGGAAGAGGAAGGAG(+AAAAAA)GTACCTGACGGCTCGGAAGCCAGAGGATCAG NGG 10 8 17 8 24 0 100 5 10
    240606154223_pvi6 ACAAACTGATCCTCTGGCTTCCGAGCCGTCAG(+AAAAAA)GTACCTCCTTCCTCTTCCCCATGTAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606154221_gscq GTTTCTCCCCCCTTCTTACTCCCTCTCCCGTG(+AAAAAA)GATCCCAGAGCCTCATGCATGCTATGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606154219_oanz AAGTGTTTTGCATAGCATGCATGAGGCTCTGG(+AAAAAA)GATCCACGGGAGAGGGAGTAAGAAGGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606154217_7elk TGCGACACCCGGGGCACCAGTTCCATTACCAG(+AAAAAA)GATCCCTGTGAGAACATTTGCATCTCTTATA NGG 10 8 17 8 24 0 100 5 10
    240606154215_6y79 CCTGTTATAAGAGATGCAAATGTTCTCACAGG(+AAAAAA)GATCCTGGTAATGGAACTGGTGCCCCGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606154214_h8eg CTAGTTAACTCACTCCCATAATGTTCCTTTGG(+AAAAAA)GTACCAGCTGTAAGCCAACACCACTCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606154213_csw9 TAATTTTTGTGAGTGGTGTTGGCTTACAGCTG(+AAAAAA)GTACCCAAAGGAACATTATGGGAGTGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606154210_0ew4 GAAAGGGTAGATGGTGCCAGTTATTTGCCCTG(+AAAAAA)GTACCTTTGGGCCCAGGAAGTTCATCACGGT NGG 10 8 17 8 24 0 100 5 10