Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152946_gh9z GGAAGGCTCCTGGCTCTAGGTCTGTGCCTCTG(+AAAAAA)GATCCACATTGGTCTTGGTCCATGTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606152945_m7b3 CAACCAGTGCCACATGGACCAAGACCAATGTG(+AAAAAA)GATCCAGAGGCACAGACCTAGAGCCAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240606152943_incr CCAAGTCTATACAGCAAGACCCTTCACTCCTG(+AAAAAA)GATCCAGAGAGAACATAATGCTTTCAATCTG NGG 10 8 17 8 24 0 100 5 10
    240606152941_nqrd AGCAGCAGATTGAAAGCATTATGTTCTCTCTG(+AAAAAA)GATCCAGGAGTGAAGGGTCTTGCTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240606152940_zfdd ATTTGTGTTCTTCCTGGGTAATCTGGTCTTAG(+AAAAAA)GATCCCTCCAGGCCTCACTACTATCAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606152938_meuv GGATAGATGCTGATAGTAGTGAGGCCTGGAGG(+AAAAAA)GATCCTAAGACCAGATTACCCAGGAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606152937_bc1o GGTGACCAATTATATGGTCAATTTTGGAGAAG(+AAAAAA)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606152936_9gt7 AAAAGGATATACCTTCTTCTCAGCACCTCATG(+AAAAAA)GTACCTTCTCCAAAATTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240606152935_9ur4 AAACAGGATGCTCTAGGTTCAGGGAAAGACTG(+AAAAAA)GATCCTAAAAACTAAGATGGAGAGCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240606152934_s9ni TCTTCTCTGTTGCTCTCCATCTTAGTTTTTAG(+AAAAAA)GATCCAGTCTTTCCCTGAACCTAGAGCATCC NGG 10 8 17 8 24 0 100 5 10