Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152843_u4ej AAGAAAATGACATGGCCTGGAGGAGATTCGGG(+AAAAAA)GTACCTTTCCTCAGTGAGCTTTGATGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606152842_4uev TTTTGGACCACATCAAAGCTCACTGAGGAAAG(+AAAAAA)GTACCCCGAATCTCCTCCAGGCCATGTCATT NGG 10 8 17 8 24 0 100 5 10
    240606152841_9aax GTTAACTGAGGGGCTAGATGTTGGCCCATGTG(+AAAAAA)GATCCCAACTTTAACATGACCAAGATTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606152839_8wyw GCCCTACCCAATCTTGGTCATGTTAAAGTTGG(+AAAAAA)GATCCACATGGGCCAACATCTAGCCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606152836_oamy AGAGAAGACACATTCCATCATTCCACCCCAGG(+AAAAAA)GTACCCTTCCACCCACAAAACTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240606152834_qzc5 TATTTTATTTTATAAGTTTTGTGGGTGGAAGG(+AAAAAA)GTACCCTGGGGTGGAATGATGGAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606152833_omeu TAAAATAAAATAAAATAAAAGGATTCCTTCTG(+AAAAAA)GATCCCAGTCCCCTTAGCTCTCTCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240606152832_qxph TGGTTGGGATGTGAGAGAGCTAAGGGGACTGG(+AAAAAA)GATCCAGAAGGAATCCTTTTATTTTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240606152829_59mg CCAGCACATTTTGAGCCCCCTCCCCACCTCCG(+AAAAAA)GATCCTGCTCCTGAGGTCCCTCAGTCCATGG NGG 10 8 17 8 24 0 100 5 10
    240606152828_8n4x ATGTACCATGGACTGAGGGACCTCAGGAGCAG(+AAAAAA)GATCCGGAGGTGGGGAGGGGGCTCAAAATGT NGG 10 8 17 8 24 0 100 5 10