Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152754_gnml TCTGTCATAATGTAGATCATGGTAGGTATGTG(+AAAAAA)GATCCCCTAAACATCTTCACATAGTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606152752_363h CATTGGAGCACACTATGTGAAGATGTTTAGGG(+AAAAAA)GATCCACATACCTACCATGATCTACATTATG NGG 10 8 17 8 24 0 100 5 10
    240606152751_3nmg TCTATTCCTGCCGAAGCTTGCCAGGGCAGGTG(+AAAAAA)GTACCCAAGAGGCTCTTCCTTTCCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606152749_u6kg CTTCTCTTCAGAGGAAAGGAAGAGCCTCTTGG(+AAAAAA)GTACCACCTGCCCTGGCAAGCTTCGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606152748_gtjq CTGTATTAAGCAGAACCCAGGTCGTTTGCTTG(+AAAAAA)GATCCACGATTTGACTTCAAGTTGTTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606152747_xkl1 TTCGAAGACAAACAACTTGAAGTCAAATCGTG(+AAAAAA)GATCCAAGCAAACGACCTGGGTTCTGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606152745_1b0k TTACAGACTGGGTGGTAAAGAGGGGCATCTTG(+AAAAAA)GTACCTGGGTCCCAGAGAGAGAAGTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606152743_uk08 CATTTTGTTTGACTTCTCTCTCTGGGACCCAG(+AAAAAA)GTACCAAGATGCCCCTCTTTACCACCCAGTC NGG 10 8 17 8 24 0 100 5 10
    240606152742_gvak TAGGGCTCACTTGTATATTAGTTTCTGAATTG(+AAAAAA)GATCCATAACTTTAAGATTAAACAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606152741_dch9 TTATCTTTTCTCTGTTTAATCTTAAAGTTATG(+AAAAAA)GATCCAATTCAGAAACTAATATACAAGTGAG NGG 10 8 17 8 24 0 100 5 10