Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152808_61bz ACAGGTAGGATTGCTGATCTGGGCACCACTGG(+AAAAAA)GATCCAAGCACTGTGTGAATCTGATCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606152807_q5b4 TGGTTTTCTAGATCAGATTCACACAGTGCTTG(+AAAAAA)GATCCCAGTGGTGCCCAGATCAGCAATCCTA NGG 10 8 17 8 24 0 100 5 10
    240606152806_9mqw AGCTGCTTCTTACTGCATAATGGCTGTTTCAG(+AAAAAA)GTACCATCACTTCACTGCTTGGCTTTTAGTC NGG 10 8 17 8 24 0 100 5 10
    240606152805_beet GCTTTGACTAAAAGCCAAGCAGTGAAGTGATG(+AAAAAA)GTACCTGAAACAGCCATTATGCAGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606152803_5zuf CTCAAATGATTGAAGTTTCATAAACTGCCTAG(+AAAAAA)GATCCTGTCAAGGCCACTGGACTCTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606152802_jhw3 GGAAAAGAACAAGAGTCCAGTGGCCTTGACAG(+AAAAAA)GATCCTAGGCAGTTTATGAAACTTCAATCAT NGG 10 8 17 8 24 0 100 5 10
    240606152800_czeg GGCCTACTGGGGGGTCCCCAAACTCAGTCCTG(+AAAAAA)GTACCACCTCAACTGTGACAATCAGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606152758_yt6k GCGAGGCTTTGCTGATTGTCACAGTTGAGGTG(+AAAAAA)GTACCAGGACTGAGTTTGGGGACCCCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606152757_w7g9 TGGAGTGTCTCTGTTCTAGAGAGAATTGGTAG(+AAAAAA)GATCCCTCAGTGTAGCATAGCCCTAAGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606152755_ocgx CCTCTACTACTTAGGGCTATGCTACACTGAGG(+AAAAAA)GATCCTACCAATTCTCTCTAGAACAGAGACA NGG 10 8 17 8 24 0 100 5 10