Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152740_k7ng ACCCTTTCTCTGACACACAGAGAGAGCTGCAG(+AAAAAA)GTACCTAGCTGAGAACCAGGGCATGGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606152738_2sab ATCCCCCTTCCCATGCCCTGGTTCTCAGCTAG(+AAAAAA)GTACCTGCAGCTCTCTCTGTGTGTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606152737_f7hp ATTATTATTATTATTACTTGTGTGTGTGTGTG(+AAAAAA)GATCCCACATCCTCCAACAGCTCCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606152736_h0oa CTTCCAACCCCTGGAGCTGTTGGAGGATGTGG(+AAAAAA)GATCCACACACACACACAAGTAATAATAATA NGG 10 8 17 8 24 0 100 5 10
    240606152734_op9j CTCAAAGCTTTGAGGAGTGAAGTTAGGAGTAG(+AAAAAA)GTACCAGTGACTACGAACTGAACATGCACTG NGG 10 8 17 8 24 0 100 5 10
    240606152733_r49a GCTCACAGTGCATGTTCAGTTCGTAGTCACTG(+AAAAAA)GTACCTACTCCTAACTTCACTCCTCAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606152732_bdz8 GTAGTGATAAACTTCTGGTGGGATAGAAAGAG(+AAAAAA)GATCCATCTTCAAACCTCAAGTCTTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606152731_s06s TCCCATCCAGGAAGACTTGAGGTTTGAAGATG(+AAAAAA)GATCCTCTTTCTATCCCACCAGAAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240606152729_naqt ATTAAGAAGAGATTTTTTTAATCTATTGAATG(+AAAAAA)GATCCTCATTTGTAAAAATTTTTAGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240606152728_a9dm AACAAAAATGGCTAAAAATTTTTACAAATGAG(+AAAAAA)GATCCATTCAATAGATTAAAAAAATCTCTTC NGG 10 8 17 8 24 0 100 5 10